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Centrum excelentnosti biochémie sacharidov GLYCOBIOS
Vedúci:
Účastnícke organizácie:
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Centrum GLYCOBIOS je zamerané na komplexný molekulárny výskum enzýmov degradujúcich a modifikujúcich komplexné sacharidy mikrobiálneho,
rastlinného a živočíšneho pôvodu. Činnosť centra zahŕňa izoláciu a biochemickú charakterizáciu enzýmov typu glykanáz,
glykozyltransferáz a esteráz, izoláciu a klonovanie príslušných génov, heterológnu expresiu, kryštalizáciu natívnych a
heterológne exprimovaných proteínov, stanovenie ich 3D štruktúry, teoretické výpočty aktívnych centier, návrhy a syntézu
špecifických inhibítorov a ich testovanie. V práci centra sa uplatňujú metodológie biochémie, proteomiky, genomiky,
teoretickej a syntetickej organickej chémie, kryštalografie a štrukturálnej chémie. Získané poznatky sú využiteľné pri
návrhu a syntéze nových liečiv, herbicídov a fungicídov, potravinárskych aditív, modifikácií polysacharidov a
biodegradácii rastlinnej hmoty.
Domovská organizácia CE GLYCOBIOS is the Chemický ústav,
Slovenská akadémia vied (SAV) v
Bratislave, Slovensko
Doba riešenia excelentného projektu GLYCOBIOS je od 01/2005 do 12/2008
Vedúci CE GLYCOBIOS je Doc. Ing. Vladimír Farkaš, DrSc.,
Chemický ústav, SAV.
Do centra v súčasnosti patrí 22 vedeckých pracovníkov, 9 doktorandov a postdoktorandov a 9 technikov. Výskum v jednotlivých oblastiach
je koordinovaný nasledujúcimi pracovníkmi:
Assoc. Prof. Vladimír Farkaš, PhD, DSc, Department of glycobiology, Institute of Chemistry, SAS (plant enzymes, molecular biology)
Peter Biely, PhD, DSc, Department of enzymology, Institute of Chemistry, SAS (microbial enzymes, biochemistry)
Igor Tvaroška, PhD, DSc, Department of structure and function of saccharides, Institute of Chemistry, SAS (molecular modelling, organic syntheses)
Jozef Ševčík, PhD, DSc, Department of structural biochemistry, Institute of Molecular Biology SAS (crystallography, protein structure)
Výskumné projekty CE GLYCOBIOS v 2005 - 2008:
a) projekty financované VEGA
(číslo projektu, názov, vedúci projektu, trvanie)
2/3079/25, Microbial hemicellulolytic glycosyl hydrolases and esterases
RNDr. Peter Biely, DrSc., 01/2003-12/2005
2/3158/25, Glycanases and their role in growth and modification of plant and fungal cell walls
Doc. Ing. Vladimír Farkaš, DrSc., 01/2003-12/2005
2/3160/25, Polygalacturonases - major enzymes of pectolytic enzyme system produced by yeasts and yeast-like microorganisms
Ing. Eva Stratilová, PhD., 01/2003-12/2005
2/5073/25, Study of capsular galactoxylomannan biosynthesis in the yeast Cryptococcus laurentii
RNDr. Nadežda Kolarova, PhD., 01/2005-12/2007
2/5074/25, Glycosyltransferases of secretory pathway from model organisms: structure-function study of GDP-L-Fuc: Asn GlcNAc alpha 1,3 fucosyltransferase (FucT 3c)
RNDr. Ján Mucha, PhD., 01/2005-12/2007
2/5075/25, Study of structure, dynamics and intermolecular complexes of biologically active saccharides using methods of NMR spectroscopy and theoretical chemistry)
Ing. Miloš Hricovíni, PhD., 01/2005-12/2007
2/6133/06, The role of glycanases in growth and modification of plant and fungal cell walls
Doc. Ing. Vladimír Farkaš, DrSc., 01/2006-12/2008
2/6128/26,
Study of honeybee antimicrobial peptides-defensisns as protection factors of honeybees and their colonies against microbial pathogens
RNDr. Jaroslav Klaudiny, PhD., 01/2006-12/2008
2/6130/26, Microbial glycoside hydrolases and esterases attacking plant cell walls
RNDr. Peter Biely, DrSc., 01/2006-12/2008
b) Research projects financed by the Agency for Science and Technology (APVT/APVV)
(project number, project leader, duration)
APVT-51-004204, Therapeutics based on an inhibition of glycosyltransferases
Ing. Igor Tvaroška, DrSc., 01/2005-12/2007
APVT-51-034504, Study of structure and properties of biologically active glycosaminoglycans in solution and their complexes with proteins
Ing. Miloš Hricovíni, PhD., 01/2005-12/2007
APVT-51-044902, Prediction of properties and functions of biological macromolecules based on computer modelling
Prof. Ing. Tomáš Bleha, DrSc. (Polymer Institute SAS), 01/2004-12/2006
APVV-51-003805, Microbial esterases cleaving linkages between saccharides and lignin in plant cell walls
RNDr. Peter Biely, DrSc., 03/2006-02/2009
c) Research projects of the State Program of Science and Research (ŠPVV) and State contracts (ŠO)
State program "Exploitation of domestic raw materials and resources"
Project no.: 2004 ŠP 26 028 0C 05
Project: Complex utilization of plant raw materials
Task 5.7.: Biopesticides based on fungi
Coordinátor: Doc. Ing. Stanislav Šilhár, CSc. (VÚP-Biocentrum, Modra)
Project leader: Doc. Ing. Vladimír Farkaš, DrSc.
Duration: 01/07/2004-31/12/2006
d) Projects of the 5th Framework Programme of the EU
Project number: QLRT-2001-02049
Heparanase inhibitors in antiangiogenic and antimetastatic cancer therapy
Acronym: HEPARANASE
Coordinator: Prof. Benito Casu (Italy)
Project leader: Ing. Miloš Hricovíni, PhD.
Duration: 10/2002-10/2005
e) Projects of the 6th Framework Programme of the EU
Project number: 005645 (FP6-2004-HRM-Marie Curie Actions-RTN)
GlycoGold: Exploration of the nature and potential of Glyco-nano-particles
Acronym: GLYCOGOLD
Coordinator: Prof. Johannis P. Kamerling (Netherlands)
Project leader: Ing. Igor Tvaroška, DrSc.
Duration: 03/2005-02/2009
Project number: 504192 (FP6-2002- HRM-Marie Curie Actions-RTN)
Functional genomics for biogenesis of plant cell walls
Acronym: WALLNET
Coordinator: Prof. Henrik Vibe Scheller (Denmark)
Project leader: Doc. Ing. Vladimír Farkaš, DrSc.
Duration: 04/2005-03/2009
Project number: 035866 (FP6-2006-HRM-Marie Curie Actions-RTN)
Contract number: MRTN-CT-2006-035866
(R) Evolutionary Catalysis
Acronym: REVCAT
Project leader: Ing. Igor Tvaroška, DrSc., Institute of Chemistry SAS
Duration: 10/2006-09/2010
f) Multilateral projects within frame of the international scientific programmes COST, INTAS, EUREKA, ESPIRIT, PHARE, NATO, UNESCO, CERN, IAEA, ESF and others)
Project number: NATO LST.CLG.979392
Differentiation, classification and evaluation of biotechnological potential of feruloyl esterases
Coordinator: Prof. Paul Christakopoulos (Greece)
Project leader: RNDr. Peter Biely, DrSc.
Duration: 05/2003-05/2005
New enzymes and selective methods for glycosidase-catalysed synthesis of bioactive glycosides and glycomimetics
Coordinator: Prof. Vladimír Křen, DrSc. (Czech Republic)
Project leader: RNDr. Peter Biely, DrSc.
Duration: 06/2001-10/2006
Project number: COST Action D29 WG No. 0008-03
Production and functionalization of hemicelluloses for sustainable advanced products
Coordinator: Prof. Tiina Maija Tenkanen (Finland)
Project leaders: RNDr. Peter Biely, DrSc. (aplicant No. 11)
Ing. Anna Ebringerová, PhD. (aplicant No. 6)
Duration: 09/2003-09/2007
Project number: COST Action 868
Biotechnical functionalisation of renewable polymeric materials
Coordinator: Prof. Georg M. Guebitz (Austria)
Project leader: RNDr. Peter Biely, DrSc.
Multilateral project (22 countries): AT, BE, CZ, DK, EG, FI, FR, DE, IT, LV, LT, NL, PL, PT, RO, RU, SK, SI, ES, SE, CH, UK
Duration: 05/2006-09/2010
g) Project within frame of inter-governmental agreements about international scientific and technical cooperation
Project number: 07 (Slovak-French agreement - program Štefánik)
Structural analyse of polysaccharides inferred from NMR in field oriented media and molecular modeling
Project leader: Ing. Igor Tvaroška, DrSc.
Duration: 01/2004-12/2005
h) Other projets financed from foreign sources
Project number: Mizutani 040013
Development of transition-state analog inhibitors of human glycosyltransferases
Project leader: Ing. Igor Tvaroška, DrSc.
Duration: 01/2004-03/2005
Project number: 436 SLK 113/9/0-1, Deutsche Forschungsgemeinschaft
The role of transglutaminases in the biogenesis of fungal cell walls
Coordintor: Prof. Widmar Tanner, Universität Regensburg, Germany
Project leader: Doc. Ing. Vladimír Farkaš, DrSc.
Duration: 07/04/2003-06/04/2006
Project number: 58-3620-2-F133, USDA
Improved utilization of corn fiber
Project leader: RNDr. Peter Biely, DrSc.
Duration: 09/2002-08/2007
PUBLIKÁCIE
Publikácie Centra excelentnosti GLYCOBIOS v 2005
(pracovníci CE sú vyznačení tučne)
| ABC
| Kapitoly vo vedeckých monografiach
|
| |
| ABC1 |
BIELY, P. - CÔTÉ, G.L. Microbial
Hemicellulolytic Carbohydrate Esterases. In HOU, Ching T. Handbook of Industrial Catalysis.
Boca Raton, Taylor & Franics Group, 2005. ISBN 0 8247 2423 2. p. 21 1 21 24. |
| |
| ADC
| Práce v časopisoch evidovaných v Current Contents
|
| |
| ADC1 |
KOLENOVÁ, K. - VRŠANSKÁ, M. - BIELY, P.
Purification and characterization of two minor endo-β-1,4 xylanases of Schizophyllum
commune. In Enzyme and Microbial Technology. Vol. 36, no. 7 (2005), p. 903-910.
(1,759 - IF2004) |
| ADC2 |
PUCHART, V. - BIELY, P. Glycosylation of
internal sugar residues of oligosaccharides catalyzed by α-galactosidase from Aspergillus
fumigatus. In Biochimica et Biophysica Acta-General Subjects. Vol. 1726, no. 2 (2005),
p. 206-216. (3,369 - IF2004) |
| ADC3 |
KREMNICKÝ, L. - BIELY, P. Unique mode of
acetylation of oligosaccharides in aqueous two-phase system by Trichoderma reesei acetyl
esterase. In Journal of Molecular Catalysis B-Enzymatic. Vol. 37, no. 1-6 (2005), p. 72-78.
(1,547 - IF2004) |
| ADC4 |
FARKAŠ, V. - STRATILOVÁ, E. - AIT MOHAND, F.
Sensitive detection of transglycosylating activity of xyloglucan endotransglycosylase/hydrolase
(XTH) after isoelectric focusing in polyacrylamide gels. In Plant Physiology and Biochemistry,
Vol. 43, (2005), p. 431-435. (IF 1,686). |
| ADC5 |
STRATILOVÁ, E. - DZÚROVÁ, M. - BREIEROVÁ,
E. - OMELKOVÁ, J. Purification and biochemical characterization of polygalacturonases produced by
Aureobasidium pullulans. In Zeitschrift für Naturforschung. Vol. 60c (2005),
p. 91-96. (IF 0.715). |
| ADC6 |
STRATILOVÁ, E. - DZÚROVÁ, M. - MALOVÍKOVÁ,
A. - OMELKOVÁ, J. Oligogalacturonate hydrolase from carrot roots. In Zeitschrift für
Naturforschung. Vol. 60c, (2005), p. 899-905. (IF 0.715). |
| ADC7 |
RAAB, M. - KOZMON, S. - TVAROŠKA, I.
Potential transition-state analogs for glycosyltransferases. Design and DFT calculations of
conformational Behavior. In Carbohydr. Res., 340, (2005), p. 1051-1057. (IF 1.451). |
| ADC8 |
TVAROŠKA, I. Structural insights into
the catalytic mechanism and transition state of glycosyltransferases using ab initio molecular
modeling. In Trends in Glycoscience and Glycotechnology, 17, (2005), p.177-190.
(1.118 - IF 2004) |
| ADC9 |
KÓŇA, J. - ZAHRADNÍK, P. - KOZMON, S. - FABIAN, V.M.F.
Role of solvent effects on nucleophilic substitution of 4H-pyran-4-one and its 2,6-dimethyl
derivative with hydroxide ion in aqueous solution: Ab initio and density functional theory studies
on a supermolecular reaction model. In J. Mol. Struc. THEOCHEM, 728 (1-3), (2005),
p.117-122. (1.007 - IF 2004) |
| ADD1 |
GAŠPERÍK, J. - HOSTINOVÁ, E. - ŠEVČÍK, J.
Acarbose binding at the surface of Saccharomycopsis fibuligera glucoamylase suggests the
presence of a raw starch-binding site. In Biologia, Bratislava, Vol. 60/Suppl. 16, (2005), p. 167-170. (IF 0.216). |
Publikácie Centra excelentnosti GLYCOBIOS v 2006
(pracovníci CE sú vyznačení tučne)
| ABC
| Kapitoly vo vedeckých monografiach
|
| |
| ABC1 |
TVAROŠKA, I. Molecular Modeling of
Retaining Glycosyltransferases. In NMR Spectroscopy and Computer Modeling of Carbohydrates
(Eds.: J.F.G. Vliegenthart and R.J. Woods), ACS Symposium Series 930, Am. Chem. Soc., Washington,
2006, DC, ISBN 0-8412-3953-3, p.285-301. |
| |
| ADC
| Práce v časopisoch evidovaných v Current Contents
|
| |
| ADC1 |
KOLENOVÁ, K. - VRŠANSKÁ, M. - BIELY, P.
Mode of action of endo-β-1,4 xylanases of families 10 and 11 on acidic xylooligosaccharides.
In Journal of Bioctechnology. Vol. 121, (2006), p. 338-345 (2,323 - IF2004) |
| ADC2 |
PUCHART, V. - GARIÉPY, M. C. - DUPONT, C.
Identification of catalytically important amino acid residues of Streptomyces lividans
acetylxylan esterase A from carbohydrate esterase family 4.
In Biochimica et Biophisica acta-Proteins and Proteomics. Vol. 1764, (2006), p. 263-274 (2,113 - IF2004) |
| ADC3 |
TAYLOR, E. J. - TURKENBURG, J. P. - GLOSTER, T. M. - VINCENT, F. - BRZOZOWSKI, A. M.
- DUPONT, C. - SHARECK, F. - CENTENO, M. S. J. - PRATES, J. A. M. - FERREIRA, L. M. A.
- FONTES, C. M. G. A. - PUCHART, V. - BIELY, P. - DAVIES, G.
Structure and activity of two metal-ion dependent acetyl xylan esterases involved in plant
cell wall degradation reveals a close similarity to peptidoglycan deacetylases,
In Journal of Biological Chemistry, 281, (2006), p.10968-10975 (6,4 - IF2004) |
| ADC4 |
MASTIHUBOVÁ, M. - SZEMESOVÁ, J. - BIELY, P.
The acetylation of p-nitrophenyl α-L-arabinofuranoside - regioselective preparation by action
of lipases.
In Bioorganic & Medicinal Chemistry. Vol. 14, (2006), p. 1805-1810. (2,018 - IF2004) |
| ADC5 |
KLUG-SANTNER, B. G. - SCHNITZHOFER, W. - VRŠANSKÁ, M.
- WEBER, J. - AGRAWAL, P. B. - NIERSTRASZ, V. A. - GUEBITZ, G. M.
Purification and characterization of a new bioscouring pectate lyase from Bacillus pumilus BK2.
In Journal of Biotechnology. 121, (3), (2006), p. 390-401 (2,323 - IF2004) |
| ADC6 |
BIELY, P. - PUCHART, V.
Recent progress in the assays of xylanolytic enzymes.
In Journal of the Science of Food and Agriculture. Vol. 86, (2006), p. 1636-1647 (0,871 - IF2004) |
| ADC7 |
ŠPÁNIKOVÁ S. - BIELY P.
Glucuronoyl esterase - a novel carbohydrate esterase from Schizophyllum commune.
In FEBS Letters Vol. 580, (2006) p. 4597-4601. |
| ADC8 |
HRMOVÁ M. - BURTON R.A. - BIELY P. - LAHNSTEIN J. - FINCHER G.B.
(1,4)-β-D-Mannan
endohydrolases from barley (Hordeum vulgare L.): Catalytic properties, primary structure,
molecular model and gene expression patterns.
In Biochemistry Journal Vol. 399, (2006), p.77-90. |
| ADC9 |
PUCHART V. - VRŠANSKÁ M. - MASTIHUBOVÁ M.
- TOPAKAS E. - VAFIADI C. - TENKANEN M. - FAULDS C.B. - CHRISTAKOPOULOS P. -
BIELY P.
Substrate and positional specificity of feruloyl esterases for monoferuloylated and
monoacetylated 4-nitrophenyl glycosides.
In Journal of Biotechnolology Vol.127, (2007), p. 235-243. |
| ADC10 |
BIELY P. - MASTIHUBOVÁ M. - PUCHART V.
The vicinal hydroxyl group is prerequisite for
metal activation of Clostridium thermocellum acetylxylan esterase.
In Biochimica et Biophysica Acta, (2007), in press. |
| ADC11 |
PUCHART V. - BIELY P.
A simple enzymatic synthesis of 4-nitrophenyl β-1,4-D-xylobioside, a chromogenic substrate
for assay and differentiation of endoxylanases.
In Journal of Biotechnolology, In press. |
| ADC12 |
SCHNITZHOFER W. - WEBER W. H.-J. -
VRŠANSKÁ M. - BIELY P. - CAVACO-PAULO A. - GUEBITZ G.M.
Purification and mechanistic characterisation of two polygalacturonases from Sclerotium rolfsii.
In Enzyme and Microbial Technology, (2007) in press |
| ADC13 |
VRŠANSKÁ M. - KOLENOVÁ K. - PUCHART V. - BIELY P.
Mode of action of glycoside hydrolase
family 5 glucuronoxylan xylanohydrolase from Erwinia chrysanthemi.
(2007) Submitted. |
| ADC14 |
KOVÁČIK, V. - BEKEŠOVÁ, S.- TVAROŠKA, I.
Positive Electrospray Ion Trap Multistage
Mass Spectrometric Fragmentation of Synthetic Analogs of Saccharide Part of
Lipopolysaccharides of Vibrio cholerae O:1.
In Journal of the American Society for Mass Spectrometry. Vol. 17, (2006), p. 749-756 (3.625-IF2005) |
| ADC15 |
KOZMON, S. - TVAROŠKA, I.
DFT Study on 3-Substituted Tetrahydropyran-2-yl Radicals.
In Collection of Czechoslovak Chemical Communications. Vol. 71, (2006), p. 1453-1469 (0.949 -IF2005) |
| ADC16 |
KOZMON, S. - TVAROŠKA, I.
Catalytic Mechanism of Glycosyltransferases:
Hybrid Quantum Mechanical/Molecular Mechanical Study of the Inverting
N-Acetylglucosaminyltransferase I.
In Journal of the American Chemical Society. Vol. 128, (2006), p. 16921-16927 (7.419 - IF2005) |
| ADC17 |
KÓŇA, J. - BRINCK, T.
Molecular dynamics simulations and quantum chemical calculations
on the mechanism of oxidation of OxyR transcription factor by hydrogen peroxide.
In Organic & Biomolecular Chemistry. Vol. 4, (2006), p. 3468-3478 (2.547 - IF2005) |
| ADC18 |
AIT MOHAND, F. - FARKAŠ, V.
Screening for hetero-transglycosylating activities in
extracts from nasturtium (Tropaeolum majus).
In Carbohydr. Res. Vol. 341 (2006), p. 577-581. (1.669 - IF2005). |
| ADC19 |
JAKUBÍKOVÁ, L. - FARKAŠ, V. - KOLAROVA, N. - NEMČOVIČ, M.
Conidiation of Trichoderma atroviride isolate during submerged cultivation in a laboratory
stirred-tank fermentor.
In Folia Microbiol., Vol. 51 (2006), p. 209-213. (0.918 - IF2005). |
| ADC20 |
MACEKOVÁ, D. - FARKAŠ, V. - KISHIDA,E. - TAKEO, K.
Ecto-glycanases and metabolic stability of the capsule in Cryptococcus neoformans.
In J. Basic Microbiol. Vol. 46 (2006), p. 470-479. (1.000 - IF2005). |
| ADC21 |
MAZÁŇ, M. - MAZÁŇOVÁ, K. - FARKAŠ, V.
Bunková stena húb - výzva pre výskum nových antimykotík. (Fungal cell wall - a challenge for
research of new antimycotics).
In Chem. Listy, Vol. 100 (2006), p. 433-439. (0.445 - IF2005). |
| ADC22 |
STRATILOVÁ, E. - DZÚROVÁ, M. -
MALOVÍKOVÁ, A. - OMELKOVÁ, Oligogalacturonate hydrolase from carrot roots.
In Zeitschrift fur Naturforschung, Vol. 60c (2006), p. 899-905. (0,602 - IF2005) |
| ADC23 |
STRATILOVÁ, E. - DZÚROVÁ, M. - BREIEROVÁ, E. - OMELKOVÁ, J.
Production and biochemical characterization of polygalacturonases produced by Aureobasidium
pullulans from forest soil.
In Annals of Microbiology, Vol. 56 (2006), p. 35-40 (0,532 - IF2005) |
| ADC24 |
ŠEVČÍK J. - HOSTINOVÁ E. - SOLOVICOVÁ A. - GAŠPERÍK J. - DAUTER Z., WILSON K.S.
Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a
raw starch binding site on the catalytic domain.
In FEBS Journal, Vol. 273 (2006), p. 2161-2171 (3.164 - IF2005) |
Publikácie Centra excelentnosti GLYCOBIOS v 2007
(pracovníci CE sú vyznačení tučne)
| |
| ADC
| Práce v časopisoch evidovaných v Current Contents
|
| |
| ADC1 |
FLODROVÁ, D.- DZÚROVÁ, M. - LIŠKOVÁ, D. -
AIT MOHAND, F. - MISLOVIČOVÁ, D.- MALOVÍKOVÁ, A. - VOBURKA, Z. -
OMELKOVÁ, J. - STRATILOVÁ, E.
Pectate Hydrolases of Parsley (Petroselinum crispum) Roots. In Z. Naturforsch.
Vol. 62c (2007), p. 382-388. |
| ADC2 |
MILLER, J. G. - FARKAŠ, V. - SHARPLES, S. C. - FRY, S. C.
O-Oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosccharides.
In Carbohydr. Res. Vol. 342 (2007), p. 44-54. |
| ADC3 |
MAZÁŇ, M. - FARKAŠ, V.
Transglutaminase-like activity participates in cell wall biogenesis in Saccharomyces cerevisiae.
In Biologia, Vol. 62 (2007), p.128-131. |
| ADC4 |
HRMOVA, M. - FARKAŠ, V. - LAHNSTEIN, J. - FINCHER, G. B.
A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic
substrates and (1,3;1,4)-β-D-glucans.
In J. Biol. Chem. Vol. 282, (2007), p. 12951-12962. |
| ADC5 |
PŘIBYLOVÁ, L. - FARKAŠ, V. - SLANINOVÁ, I. - DE MONTIGNY, J. - SYCHROVÁ, H.
Differences in osmotolerant and cell-wall properties of two Zygosaccharomyces rouxii strains.
In Folia Microbiol. Vol. 52 (2007), p. 241-245. |
| ADC6 |
LI X.-L. - ŠPÁNIKOVÁ S. - DEVRIES R.P. - BIELY P.
Identification of genes encoding microbial glucuronoyl esterases.
In FEBS Lett., Vol. 581 (2007), p. 4029-4035. |
| ADC7 |
ŠPÁNIKOVÁ S. - POLÁKOVÁ M. - JONIAK D. - HIRSCH J. - BIELY P.
Synthetic esters recognized by glucuronoyl esterase from Schizophyllum commune.
In Arch. Microbiol., Vol.188 (2007), p. 185-189. |
| ADC8 |
VRŠANSKÁ M. - KOLENOVÁ K. - PUCHART V. - BIELY P.
Mode of action of glycoside hydrolase family 5 glucuronoxylan xylanohydrolase from
Erwinia chrysanthemi.
In FEBS Journal, Vol.274 (2007), p. 1666-1677. |
| ADC9 |
BIELY P. - MASTIHUBOVÁ M. - PUCHART V.
The vicinal hydroxyl group is prerequisite for metal activation of Clostridium
thermocellum acetylxylan esterase.
In Biochim. Biophys. Acta, Vol. 1770 (2007), p. 565-570. |
| ADC10 |
PUCHART V. - VRŠANSKÁ M. - MASTIHUBOVÁ M. - TOPAKAS E.
- VAFIADI C. - TENKANEN M. - FAULDS C.B. - CHRISTAKOPOULOS P. - BIELY P.
Substrate and positional specificity of feruloyl esterases for monoferuloylated and
monoacetylated 4-nitrophenyl glycosides.
In J. Biotechnol.,Vol. 127 (2007), p. 235-24. |
| ADC11 |
PUCHART V. - BIELY P.
A simple enzymatic synthesis of 4-nitrophenyl β-1,4-D-xylobioside, a chromogenic
substrate for assay and differentiation of endoxylanases.
In J. Biotechnol.,Vol. 128 (2007) p. 276-286. |
| ADC12 |
SCHNITZHOFER W. - WEBER W. H.-J. - VRŠANSKÁ M. - BIELY P.
- CAVACO-PAULO A. - GUEBITZ G.M.
Purification and mechanistic characterisation of two polygalacturonases from Sclerotium rolfsii.
In Enzyme Microb. Technol.,Vol. 40 (2007), p. 1739-1747. |
Publikácie Centra excelentnosti GLYCOBIOS v 2008
(pracovníci CE sú vyznačení tučne)
| |
| ADC
| Práce v časopisoch evidovaných v Current Contents
|
| |
| ADC1 |
BIELY, P. - LEATHERS, T.D. - CZISZÁROVÁ, M.
- VRŠANSKÁ, M. - COTTA, M.A.
Endo-β-1,4-xylanase inhibitors in leaves and roots of germinated maize.
In Journal of Cereal Science. 47: 27-32 (2008). |
| ADC2 |
LI, X.-L. - SKORY, C.D. - COTTA, M.A. - PUCHART, V. -
BIELY, P. Novel family of carbohydrate esterases, based on identification
of the Hypocrea jecorina acetyl esterase gene. In Applied and Environmental Microbiology. 74: 7482-7489 (2008). |
| ADC3 |
KYLLI, P. - NOUSIAINEN, P. - BIELY, P. - SIPILÄ, J. -
TENKANEN, M. - HEINONEN, M. Antioxidant potential of hydroxycinnamic acid glycoside esters.
Journal of Agricultural and Food Chemistry. 56: 4797-4805 (2008). |
| ADC4 |
PUCHART, V. - BIELY, P.
Simultaneous production of endo-β-1,4-xylanase and branched xylooligosaccharides by Thermomyces lanuginosus.
In Journal of Biotechnology. 137: 34-43 (2008). |
| ADC5 |
VRŠANSKÁ, M. - NERINCKX, W. - CLAEYSSENS, M.
- BIELY, P. An alternative approach for the synthesis of fluorogenic substrates
of endo-β-(1→4)-xylanases and some applications. In Carbohydrate Research. 343: 541-548 (2008). |
| ADC6 |
WOOD, S.J. - LI, X.-L. - COTTA, M.A. - BIELY, P.
- DUKE, N.E. - SCHIFFER, M. - POKKULURI, P.R. Crystallization and preliminary X-ray diffraction analysis
of the glucuronoyl esterase catalytic domain from Hypocrea jecorina.
In Acta Crystallographica Section F. F64: 255-257 (2008). |
| ADC7 |
FAIK, A. – SHIPP, M. – NADELLA, R. – GAO, H. – FARKAŠ, V.
– SIGRIST, H.: Glyco-array technology for efficient monitoring of plant cell wall glycosyltransferase activities.
In Glycoconj. J. 25: 49-58 (2008). |
| ADC8 |
KOSÍK, O. – FARKAŠ, V.:
One-pot synthesis of xyloglucan oligosaccharides fluorescently labeled with sulforhodmine.
In Anal. Biochem. 375: 232-236 (2008). |
| ADC9 |
NEMČOVIČ, M. – JAKUBÍKOVÁ, L.
– VIDEN, I. – FARKAŠ, V.: Induction of conidiation by endogenous volatile
compounds in Trichoderma spp. In FEMS Microbiol. Lett. 284: 231-236 (2008). |
| ADC10 |
MARKUS, S. E. - VERHERTBRUGGEN, Y. – HERVÉ, C. – ORDAZ-ORTIZ, J. J.
– FARKAŠ, V. – PEDERSEN, H. – WILLATS, W. G. T. – KNOX, P.:
Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls.
In BMC Plant Biol. 8: 60, doi: 10.1186/1471-2229-8-60, (2008). |
| ADC11 |
HRMOVA, M. - FARKAŠ, V. - HARVEY, A. J.
– LAHNSTEIN, J. – WISCHMANN, B. – KAEWTHAI, N. – EZCURRA, I. – TEERI, T. – FINCHER, G. B.:
Xyloglucan xyloglucosyl transferases from barley (Hordeum vulgare L.): Substrate specificity,
catalytic mechanism, molecular structure and characterization of the acceptor substrate binding site.
In FEBS J. 276: 437-456 (2009). |
| ADC12 |
CABIB, E. – FARKAŠ, V. – KOSÍK, O.
– BLANCO, N. – ARROYO, J. – McPHIE, P.: Assembly of the yeast cell wall: Crh1p and Crh2p act as
transglycosylases in vivo and in vitro. In J. Biol. Chem. 283: 29859-29872 (2008). |
| ADC13 |
FARKAŠ, V. - TAKEO, K. - MACEKOVÁ, D.
- OHKUSU, M. - YOSHIDA, S. – SIPICZKI, M.: Secondary cell wall formation in Cryptococcus neoformans
as a rescue mechanism against acid-induced autolysis. In FEMS Yeast Res. 9: 311-320 (2009). |
| ADC14 |
STRATILOVÁ, E. - DZÚROVÁ M.
- BREIEROVÁ, E. - MALOVÍKOVÁ, A. - OMELKOVÁ, J.:
The life style of Aureobasidium pullulans and the multiple forms of its polygalacturonase.
In Biologia, 61: 257-262 (2008). |
| ADC15 |
GARAJOVÁ, S. - FLODROVÁ, D. - AIT-MOHAND, F.
- FARKAŠ, V. - STRATILOVÁ, E.: Characterization of two partially
purified xyloglucan endotransglycosylases from parsley (Petroselinum crispum) roots. In Biologia 63 : 313-319 (2008). |
| ADC16 |
KOSÍK, O. – FARKAŠ, V.:
Effect of the label on the affinity of xyloglucan endotransglycosylase (XET) towards oligosaccharide acceptors in vitro (submitted). |
| ADC17 |
KOSÍK, O. – AUBURN, R. – RUSSEL, S. - HRMOVÁ, M.
– FARKAŠ, V.: Polysaccharide glycochip for screening of transglycosylase activities in plant extracts. (submitted). |
| ADC18 |
SIHELNIKOVA, L. - KOZMON, S.
- TVAROSKA, I.: DFT and docking study of potential transition state analogue inhibitors
of glycosyltransferases. In Coll. Czechoslov. Chem. Commun. 73: 591 - 607 (2008). |
| ADC19 |
SIHELNIKOVA, L. - TVAROSKA, I.:
Elucidation of the Au-S bond in a passivated gold cluster through density functional theory calculations'.
In J. Phys. Condens. Matter 20: a. 64214 (2008). |
| ADC20 |
SPIWOK, V. - KRALOVA, B.
- TVAROSKA, I.: Continuous metadynamics in essential coordinates as a tool for
free energy modelling of conformational changes. In J. Molec. Modelling 14: 995 - 1002 (2008). |
| ADC21 |
SPIWOK, V. - TVAROŠKA, I.:
The Confromational free energy surface of α-N-acetylneuraminic acid: an interplay between hydrogen bondong
and solvation. In J. Phys.Chem. B (submitted). |
| ADC22 |
HIRSCH, J. - KOÓŠ, M.
- TVAROŠKA, I.: Synthesis of saccharide precursors for preparation of the potential
inhibitors of glycosyltranferases. In Chem. Papers. (submitted). |
| |
| PV
| Prihlášky vynálezov
|
| |
| 1 |
ŠPÁNIKOVÁ, S. - DE VRIES, R. P. - BIELY, P.:
Glucuronoylesterase from Schizophyllum commune, method of its preparation and use for degradation of
biological materials. Slovak patent application no. PP 95-2005.
|
| 2 |
FARKAŠ, V. - NEMČOVIČ, M. – JAKUBÍKOVÁ,
L.- ŠUBÍKOVÁ, V. – DRÍMAL, J. – JANITOR, A. – KUNCA, A. – LEONTOVIČ, R.: Strains of the micromycete Trichoderma
and preparation for plant protection containing the said. Slovak patent application no.PP 5108-2008.
|
| 3 |
KOSÍK, O. - FARKAŠ, V.: High-throughput
method for determining the activity of transglycoylases. Slovak patent application no. PP .
|
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