About the Institute of Chemistry, SAS People at the Institute of Chemistry, SAS Research groups at the Institute of Chemistry, SAS Research at the Institute of Chemistry, SAS Products of the Institute of Chemistry, SAS






Vladimír FARKAŠ
PhD, DSc, Assoc. Prof.
Senior Research Scientist
Head

Laboratory of Polysaccharide Metabolism

Tel.: +421-2-59410216
Fax.: +421-2-59410222
Research Interests
  • Plant and fungal cell walls, their structre, biosynthesis and biodegradation. Attention is focused to hydrolases with transglycosylating activity, such as plant xyloglucan endo-transglycosylase (XET).
  • Light-induced fungal development. As the model organism, Trichoderma viride is used.
  • Selected Publications (last five years, full list available on request)
    FARKAŠ, V. - AIT MOHAND, F. - STRATILOVÁ E.:
    Sensitive detection of transglycosylating activity of xyloglucan endotransglycosylase/hydrolase (XTH) after isoelectric focusing in polyacrylamide gels.
    Plant Physiol. Biochem. 43, 431-435 (2005).
    AIT MOHAND, F. - FARKAŠ, V.:
    Screening for hetero-transglycosylating activities in extracts from nasturtium (Tropaeolum majus).
    Carbohydr. Res. 341, 577-581 (2006).
    JAKUBÍKOVÁ, L. - FARKAŠ, V. - KOLAROVA, N. - NEMČOVIČ, M.:
    Conidiation of Trichoderma atroviride isolate during submerged cultivation in a laboratory stirred-tank fermentor. Folia Microbiol. 51, 209-213 (2006).
    MACEKOVÁ, D. - FARKAŠ, V. - KISHIDA,E. - TAKEO, K.:
    Ecto-glycanases and metabolic stability of the capsule in Cryptococcus neoformans.
    J. Basic Microbiol. 46: 470-479 (2006).
    MILLER, J. G. - FARKAŠ, V. - SHARPLES, S. C. - FRY, S. C.:
    O-Oligosaccharidyl-1-amino-1-deoxyalditols as intermediates for fluorescent labelling of oligosccharides.
    Carbohydr. Res. 342: 44-54 (2007).
    MAZÁŇ, M. - FARKAŠ, V.:
    Transglutaminase-like activity participates in cell wall biogenesis in Saccharomyces cerevisiae.
    Biologia 62: 128-131 (2007).
    HRMOVA, M. - FARKAŠ, V. - LAHNSTEIN, J. - FINCHER, G. B.:
    A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates and (1,3;1,4)-beta-D-glucans.
    J. Biol. Chem. 282:12951-12962 (2007).
    PŘIBYLOVÁ, L. - FARKAŠ, V. - SLANINOVÁ, I. - DE MONTIGNY, J. - SYCHROVÁ, H.:
    Differences in osmotolerant and cell-wall properties of two Zygosaccharomyces rouxii strains.
    Folia Microbiol. 52: 241-245 (2007).
    FAIK, A. - SHIPP, M. - NADELLA, R. - GAO, H. - FARKAŠ, V. - SIGRIST, H.:
    Glyco-array technology for efficient monitoring of plant cell wall glycosyltransferase activities.
    Glycoconjug. J. 25: 49-58 (2008).
    KOSÍK, O. - FARKAŠ, V.:
    One-pot synthesis of xyloglucan oligosaccharides fluorescently labeled with sulforhodmine.
    Anal. Biochem. 375: 232-236 (2008).
    NEMČOVIČ, M. - JAKUBÍKOVÁ, L. - VIDEN, I. - FARKAŠ, V.:
    Induction of conidiation by endogenous volatile compounds in Trichoderma spp.
    FEMS Microbiol. Lett. 284: 231-236 (2008)
    MARKUS, S. E. - VERHERTBRUGGEN, Y. - HERVÉ, C. - ORDAZ-ORTIZ, J. J. - FARKAŠ, V. - PEDERSEN, H. - WILLATS, W. G. T. - KNOX, P.:
    Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls.
    BMC Plant Biol. 8: 60, doi: 10.1186/1471-2229-8-60.
    GARAJOVÁ, S. - FLODROVÁ, D. - AIT-MOHAND, F. - FARKAŠ, V. - STRATILOVÁ, E.:
    Characterization of two partially purified xyloglucan endotransglycosylases from parsley (Petroselinum crispum) roots.
    Biologia 63 : 313-319 (2008).
    HRMOVA, M. - FARKAŠ, V. - HARVEY, A. J. - LAHNSTEIN, J. - WISCHMANN, B. - KAEWTHAI, N. - EZCURRA, I. - TEERI, T. - FINCHER, G. B.:
    Xyloglucan xyloglucosyl transferases from barley (Hordeum vulgare L.): Substrate specificity, catalytic mechanism, molecular structure and characterization of the acceptor substrate binding site.
    FEBS J. (in press).
    CABIB, E. - FARKAŠ, V. - KOSÍK, O. - BLANCO, N. - ARROYO, J. - McPHIE, P.:
    Assembly of the yeast cell wall: Crh1p and Crh2p act as transglycosylases in vivo and in vitro.
    J. Biol. Chem. 283: 29859-29872 (2008).
    FARKAŠ, V. - TAKEO, K. - MACEKOVÁ, D. - OHKUSU, M. - YOSHIDA, S. - SIPICZKI, M.:
    Secondary cell wall formation in Cryptococcus neoformans as a rescue mechanism against acid-induced autolysis.
    FEMS Yeast Res., 9: 311-320 (2009).
    KOSÍK, O. - AUBURN, R. - RUSSELL, S. - STRATILOVÁ, E. - GARAJOVÁ, S. - HRMOVA, M. - FARKAŠ, V.:
    Polysaccharide microarrays for high-throughput screening of transglycosylase activities in plant extracts.
    Glycoconj. J. 27: 79-87 (2110). doi: 10.1007/s10719-009-9271-8.
    STRATILOVÁ, E. - AIT MOHAND, F. - ŘEHULKA, P. - GARAJOVÁ, S. - FLODROVÁ, D. - ŘEHULKOVÁ, H. - FARKAŠ, V.:
    Xyloglucan Endotransglycosylases (XETs) from germinating nasturtium (Tropaeolum majus) seeds: Isolation and characterization of the major form.
    Plant Physiol. Biochem. 48: 207-215 (2010), doi: 10.1016/j.plaphy.2010.01.016.
    VAAJE-KOLSTAD, G. - FARKAŠ, V. - FINCHER, G. B. - HRMOVA, M.:
    Barley xyloglucan xyloglucosyl transferases bind xyloglucan-derived oligosaccharides in their acceptor-binding regions in multiple conformational states.
    Arch. Biochem. Biophys. 496: 61-68 (2010). doi: 10.1016/j.abb.2010.01.011.
    KOSÍK, O. - GARAJOVA, S. - STRATILOVÁ, E. - FARKAŠ, V.:
    Effect of the label of oligosaccharide acceptors on kinetic parameters of nasturtium seed xyloglucan endotransglycosylase/hydrolase
    (Carbohydr. Res., submitted)
    VAAJE-KOLSTAD, G. - FARKAŠ, V. - HRMOVA, M. - FINCHER, G. B.:
    Xyloglucan xyloglucosyl transferases from barley (Hordeum vulgare L.) bind oligomeric and polymeric xyloglucan molecules in their acceptor binding sites.
    Biochim. Biophys. Acta 1800: 674-684 (2010), doi: 10.1016/j.bbagen.2010.04.001
    Institute of Chemistry
    Slovak Academy of Sciences
    webmaster@chem.sk
    2012